Modifying Enzymes
Discovery Life Sciences HUMAN CYP3A+P450 REDUCTASE
Recombinant enzyme prepared from insect cells infected with baculovirus containing desired cDNAs. CYP3A4 is on average the most abundant P450 in human liver. It is considered the most important P450 for drug metabolism, accounting for the metabolism of 50% or more of prescribed medications. CYP3A4 is also a major isoform in the intestine and can contribute significantly to oral first-pass metabolism of drugs. CYP3A4 metabolizes cortisol, nifedipine, cyclosporin, testosterone, estrogen, (R)-warfarin, gestodene, ketoconazole, miconazole, dapsone, FK-506, quinidine, lovastatin, erythromycin, and numerous other drugs. It is involved in many clinically important drug-drug interactions. CYP3A4 activates aflatoxin B1-related Carcinogens and senecionine. CYP3A4 protein levels can be induced several fold in the liver by ligands of CAR and PXR (e.g., phenobarbital, rifampin, and phenytoin). Contains Human Oxidoreductase and cytochrome b5
New England Biolabs, Inc. PNGase A – 150 units
PNGase A cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and short complex oligosaccharides such as those found in plant and insect cells from N-linked glycoproteins and glycopeptides. PNGase A differs from PNGase F in that it cleaves N-linked glycans with or without (1,3)-linked core fucose residues.
Chem-Impex International, Inc. Pepsin 1:10000 | 9001-75-6 | MFCD00081840 | 100G
Pepsin 1:10000, 9001-75-6, MFCD00081840, 100G
New England Biolabs, Inc. Bacteroides Heparinase II – 200 units
Bacteroides Heparinase II (also called Heparin Lyase II) is cloned from Bacteroides eggerthii. It is a low specificity enzyme that is active on both heparin and heparan sulfate. Bacteroides Heparinase II cleaves the glycosidic bond between N-sulfated and glucuronic or iduronic acid residues. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. When used alone this enzyme rarely yields complete depolymerization of a polysaccharide chain, however disaccharide analysis is enhanced when used in combination with Heparinase I and III.
New England Biolabs, Inc. β1-4 Galactosidase S - 2000 units
b1-4 Galactosidase S is a highly specific exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing b1-4 linked galactose residues from oligosaccharides.
Supply Solutions Roche - BSA, 20mg, SQ for Molecular Biology; from bovine serum; pkg of 1 mL (20 mg/ml)
Roche - BSA, 20mg, SQ for Molecular Biology; from bovine serum; pkg of 1 mL (20 mg/ml)
New England Biolabs, Inc. TaqI Methyltransferase – 1000 units
TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA.
New England Biolabs, Inc. Klenow Fragment (3'-5' exo-) – 1000 units
Klenow Fragment (3' to 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5' to 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3' to 5' exonuclease activity.
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 1600 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
New England Biolabs, Inc. Topoisomerase I (E. coli) – 100 units
Topoisomerase I (E. coli) catalyzes the relaxation of negatively supercoiled DNA. Topoisomerase I has also been implicated in knotting and unknotting DNA and in linking complementary rings of single-stranded DNA into double-stranded rings. The intact holoenzyme is a 97 kDa protein.
New England Biolabs, Inc. Taq DNA Ligase – 2000 units
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37 C - 75 C).
New England Biolabs, Inc. α2-3 Neuraminidase S - 2000 units
a2-3 Neuraminidase S is a highly specific exoglycosidase that catalyzes the hydrolysis of a2-3 linked N-acetylneuraminic acid residues from oligosaccharides and glycoproteins.
Supply Solutions Liberase™ TH Research Grade, 2x5mg
Liberase TH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other and with a high concentration of Thermolysin, a non-clostridial neutral protease.
New England Biolabs, Inc. T7 Exonuclease – 1000 units
T7 Exonuclease acts in the 5' to 3' direction, catalyzing the removal of 5' mononucleotides from linear or nicked duplex DNA.
New England Biolabs, Inc. Endonuclease III (Nth) – 1000 units
Endonuclease III (Nth) protein from E. coli acts as both N-glycosylase and a AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating a basic (AP site). The AP-lyase activity of the enzyme cleaves 3 to the AP site leaving a 5' phosphate and a 3' ring opened sugar. Some of the damaged bases recognized and removed by Endouclease III include urea, 5, 6 dihydroxythymine, thymine glycol, 5-hydroxy-5 methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihdrothimine and methyltartronylurea.